Dr Sai-Kiang Lim

Lim Sai Kiang

Principal Investigator, Lineage Restriction in Stem Cells

Research Associate Professor (Joint), Dept. of Surgery, Yong Loo Lin School of Medicine, National University of Singapore

embryonic stem cells, mesodermal and endodermal stem cells, cell therapeutics, biologics

Email: saikiang.lim@imb.a-star.edu.sg

Biography

Dr Sai-Kiang Lim graduated with B.Sc (Hons in Biochemistry) from National University of Singapore in 1985. She did her PhD thesis on the metabolism of thalassemic mRNA under Prof Lynne Maquat (Dept. of Human Genetics, Roswell Park Cancer Institute), and was awarded the Most Meritorious Student Research Award by Sigma Xi Society in 1989, NY State Predoctoral Fellowship (1989-91) and a PhD (Molecular Biology) from SUNY at Buffalo in 1992. In 1992, she started postdoctoral training on erythroid differentiation with Prof Frank Constantini (Dept. of Genetics and Development College of Physicians & Surgeons, Columbia University) first as a Cooley’s Anemia Foundation Research Fellow (1992-94) and then a Leukemia Society of America Special Fellow (1994-96). After postdoctoral training, she led independent research groups at NUMI, NUS (1996-2001), Genome Institute of Singapore (2002-2007) and then Institute of Medical Biology (2007). Her research focus has always been disease-related with emphasis on the elucidation of the underlying molecular and cellular mechanisms in diseases and development of therapies. Together with her collaborator, Dr Guodong Li (NUMI, NUS), they were awarded the “Novel & Newsworthy Top Ten in Cell Biology in 2005" by the American Society for Cell Biology (ASCB) for their work on the ‘Generation and Verification of Functional Insulin-producing Cells Derived from Embryonic Progenitor Cells’.

Insulin & C-peptide immunostaining
of ERoSHK cells

Representative Publications

1. Lian Q., Yeo K.S., Que J., Tan E.K.Y., Yu F, Yin Y, Salto-Tellez M, El Oakley R.M., Lim S.-K* (2006). Establishing clonal cell lines with endothelial-like potential from CD9hi, SSEA-1- cells in embryonic stem cell-derived embryoid bodies. PLoS ONE1:e6.

2. Lian Q., Lye E. Yeo K.S., Tan E.K.Y., Salto-Tellez M., Liu T.M., Palanisamy N., El Oakley R.M., Lee E.H., Lim B., Lim S.-K. * (2007) Derivation of Clinically Compliant MSCs from CD105+, CD24- Differentiated Human ESCs. Stem Cells 25(2):425-36.

3. Sze S.K., de Kleijn D.P.V., Lai R.C., Tan E.K.Y., Zhao H., Yeo K.S., Low T.Y., Lian Q., Lee C.N., Mitchell W., El Oakley R.M., and Lim S.-K. * (2007) Elucidating the secretion proteome of human ESC-derived mesenchymal stem cells. Molecular and Cellular Proteomics 6:1680-1689.

4. Que J., Lian Q., El Oakley R.M., Lim B., Lim S.-K. * (2007) PI3 K/Akt/mTOR regulates self-renewal and endothelial differentiation in lineage restricted RoSH stem cell lines. J Mol Signal. Sep 25;2(1):9.

5. Timmers L., Lim S.-K.*; Arslan F.; Armstrong J.S., Hoefer I.E., Doevendans P.A.; Piek J.J., El Oakley R.M., Choo A., ; Lee C.N., Pasterkamp G., de Kleijn D.P.V. * (2008) Reduction of myocardial infarct size by human mesenchymal stem cell conditioned medium. Stem Cell Research 1:129–137.

6. Choo A.; Ngo A. S.; Ding V.; Oh S.; Lim S.-K.(2008), Autogeneic feeders for the culture of undifferentiated human embryonic stem cells in feeder and feeder-free conditions. Methods Cell Biol 86:15-28.

7. van Keulen, J.K, Timmers, L., van Kuijk, L.P., Retnam, L., Hoefer, I., Pasterkamp, G., Lim, S.-K., de Kleijn, D.P.V. The Nuclear Factor-kappa B p50 subunit is involved in flow-induced outward arterial remodelling. Atherosclerosis (in press) <http://dx.doi.org/10.1016/j.atherosclerosis.2008.05.049> .

8. Chee S.G., Guo T., Zhang H., Lim S.-K., and Sze S.K.*(2008) A Comparative Study of Electrostatic Repulsion-Hydrophilic Interaction Chromatography (ERLIC) versus SCX-IMAC-Based Methods for Phosphopeptide Isolation/Enrichment. Journal of Proteome Research 7 (11):4869–4877.

9. Li, G.D. *, Luo R., Zhang J., Yeo K.S., Lian Q., Xie F., Tan E.K.Y., Caille D., Que J., Kon O.L., Salto-Tellez M, Meda, P., and Lim S.-K.* (2009) Generating mESC-derived Insulin-producing Cell Lines through an Intermediate Lineage-restricted Progenitor Line. Stem Cell Research 2:41–55.

10. Li, G.D. *, Luo, R., Zhang, J., Yeo K.S., Xie, F., Tan E.K.Y., Caille D., Que J., Kon, O.L., Salto-Tellez M, Meda P., and Lim S.-K.* (2009) Derivation of functional insulin-producing cell lines from primary mouse embryo culture. Stem Cell Research 2:29–40.

* corresponding author